榈濋檷閳ｇ礌(CT)ELISA妾㈡脯(c猫)瑭﹀姂鐩掑(sh铆)椹�(y脿n)鍘熺悊
2022骞�03鏈�18鏃�| 璩囨枡椤炲瀷 | docx鏂囦欢 | 璩囨枡澶у皬 | 19397 |
| 涓嬭級娆℃暩(sh霉) | 17 | 璩囨枡鍦栫墖 | 銆愰粸(di菐n)鎿婃煡鐪嬨�� |
| 涓� 鍌� 浜� | 涓婃捣鐮旂敓鐢熷寲瑭﹀姂鏈夐檺鍏徃 | 闇�瑕佺⿳鍒� | 0 |
| 闂�(gu膩n) 閸� 瑭� | 榈濋檷閳ｇ礌(CT)ELISA妾㈡脯(c猫)瑭﹀姂鐩�,榈濋檷閳ｇ礌(CT)ELISA妾㈡脯(c猫)瑭﹀姂鐩�,榈濋檷閳ｇ礌(CT)ELIS | ||
- 銆愯硣鏂欑啊(ji菐n)浠嬨��
榈濋檷閳ｇ礌(CT)ELISA妾㈡脯(c猫)瑭﹀姂鐩掕(shu艒)鏄庢浉
鍎�(y艒u)鍕�(sh矛)锛�
1銆�鎶楅珨----楂樻晥銆侀潏鏁忋�佺壒鐣版�уソ
2銆佽(gu墨)鑼冨寘琚搷浣�----鍚搁檮鍧囧嫽銆佸惛闄勬�уソ銆佺┖鐧藉�间綆銆佸瓟搴曢�忔槑搴﹂珮
3銆佸厛閫�(j矛n)鐨勫劒(y艒u)鍖栨柟妗�----閲嶅京(f霉)鎬ч珮锛屽彲闈犳�у挤(qi谩ng)
4銆侀珮妯�(bi膩o)婧�(zh菙n)鎶�琛�(sh霉)鏈嶅嫏(w霉)瑕佹眰锛氶潏鏁忓害楂橈紝鐗圭暟鎬у挤(qi谩ng)
5銆侀仼鐢ㄨ寖鍦嶅唬锛氳婕裤�佽娓呫�佺祫绻斿嫽婕挎恫銆佺窗(x矛)鑳炲煿椁�(y菐ng)涓婃竻娑层�佸翱娑茬瓑澶氱ó椤炲瀷鐨勬ǎ鏈�
妾㈡脯(c猫)鍘熺悊
瑭﹀姂鐩掗噰鐢ㄩ洐鎶楅珨涓�姝ュぞ蹇冩硶閰惰伅(li谩n)鍏嶇柅鍚搁檮瑭﹂(y脿n)锛圗LISA锛夈�傚線闋�(y霉)鍏堝寘琚祫鑳猴紙HIS锛夋姉楂旂殑鍖呰寰瓟涓紝渚濇鍔犲叆妯�(bi膩o)鏈�佹(bi膩o)婧�(zh菙n)鍝併�丠RP妯�(bi膩o)瑷樼殑妾㈡脯(c猫)鎶楅珨锛岀稉(j墨ng)閬�(gu貌)婧偛骞�*娲楁粚銆傜敤搴曠墿TMB椤壊锛孴MB鍦ㄩ亷(gu貌)姘у寲鐗╅叾鐨勫偓鍖栦笅杞�(zhu菐n)鍖栨垚钘�(l谩n)鑹诧紝骞跺湪閰哥殑浣滅敤涓嬭綁(zhu菐n)鍖栨垚绲傜殑榛冭壊銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑鐨勭祫鑳猴紙HIS锛夊憟姝ｇ浉闂�(gu膩n)銆傜敤閰舵(bi膩o)鍎�鍦�450nm 娉㈤暦(zh菐ng)涓嬫脯(c猫)瀹氬惛鍏夊害锛圤D 鍊硷級锛岃▓(j矛)绠楁ǎ鍝佹績搴︺��
妯ｅ搧鏀堕泦銆佽檿鐞嗗強淇濆瓨鏂规硶
1. 琛�娓咃細浣跨敤涓嶅惈鐔卞師鍜屽収(n猫i)姣掔礌鐨勮│绠★紝鎿嶄綔閬�(gu貌)绋嬩腑閬垮厤浠讳綍绱�(x矛)鑳炲埡婵�锛屾敹闆嗚娑插悗锛�3000杞�(zhu菐n)闆㈠績10鍒嗛悩灏囪娓呭拰绱呯窗(x矛)鑳炶繀閫熷皬蹇冨湴鍒嗛洟銆�
2. 琛�婕匡細EDTA銆佹妾吀楣芥垨鑲濈礌鎶楀嚌銆�3000杞�(zhu菐n)闆㈠績30鍒嗛悩鍙栦笂娓呫��
3. 绱�(x矛)鑳炰笂娓呮恫锛�3000杞�(zhu菐n)闆㈠績10鍒嗛悩鍘婚櫎椤嗙矑鍜岃仛鍚堢墿銆�
4. 绲勭箶鍕绘伎锛氬皣绲勭箶鍔犲叆閬╅噺鐢熺悊楣芥按鎼楃銆�3000杞�(zhu菐n)闆㈠績10鍒嗛悩鍙栦笂娓呫��
5. 淇濆瓨锛氬鏋滄ǎ鏈敹闆嗗悗涓嶅強鏅�(sh铆)妾㈡脯(c猫)锛岃珛(q菒ng)鎸変竴娆＄敤閲忓垎瑁濓紝鍑嶅瓨浜�-20鈩冿紝閬垮厤鍙嶅京(f霉)鍑嶈瀺锛屽湪瀹ゆ韩涓嬭В鍑嶅苟纰轰繚妯ｅ搧鍧囧嫽鍦板厖鍒嗚В鍑嶃��
鎿嶄綔姝ラ
寰炲婧钩琛�20min鍚庣殑閶佺當琚嬩腑鍙栧嚭鎵�闇�鏉挎锛屽墿浣欐澘姊濈敤鑷皝琚嬪瘑灏佹斁鍥�4鈩冦��
瑷�(sh猫)缃(bi膩o)婧�(zh菙n)鍝佸瓟鍜屾ǎ鏈瓟锛屾(bi膩o)婧�(zh菙n)鍝佸瓟鍚勫姞涓嶅悓婵冨害鐨勬(bi膩o)婧�(zh菙n)鍝�50渭L锛�
妯ｆ湰瀛斿厛鍔犲緟娓�(c猫)妯ｆ湰10渭L锛屽啀鍔犳ǎ鏈█閲嬫恫40渭L锛涚┖鐧藉瓟涓嶅姞銆�
闄ょ┖鐧藉瓟澶栵紝妯�(bi膩o)婧�(zh菙n)鍝佸瓟鍜屾ǎ鏈瓟涓瘡瀛斿姞鍏ヨ荆鏍归亷(gu貌)姘у寲鐗╅叾锛圚RP锛夋(bi膩o)瑷樼殑妾㈡脯(c猫)鎶楅珨100渭L锛岀敤灏佹澘鑶滃皝浣忓弽鎳�(y墨ng)瀛旓紝37鈩冩按娴撮崑鎴栨亽婧婧偛60min銆�
妫勫幓娑查珨锛屽惛姘寸礄涓婃媿骞诧紝姣忓瓟鍔犳豢娲楁粚娑诧紝闈滅疆1min锛岀敥鍘绘礂婊屾恫锛屽惛姘寸礄涓婃媿骞诧紝濡傛閲嶅京(f霉)娲楁澘5娆★紙涔熷彲鐢ㄦ礂鏉挎(j墨)娲楁澘锛夈��
姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50渭L锛�37鈩冮伩鍏夊鑲�15min銆�
姣忓瓟鍔犲叆绲傛娑�50渭L锛�15min鍏�(n猫i)锛屽湪450nm娉㈤暦(zh菐ng)铏曟脯(c猫)瀹氬悇瀛旂殑OD鍊笺��
绲�(ji茅)鏋滃垽鏂�
绻埗妯�(bi膩o)婧�(zh菙n)鏇茬窔锛氬湪Excel宸ヤ綔琛ㄤ腑锛屼互妯�(bi膩o)婧�(zh菙n)鍝佹績搴︿綔姗潗妯�(bi膩o)锛屽皪(du矛)鎳�(y墨ng)OD鍊间綔绺卞潗妯�(bi膩o)锛岀躬鍒跺嚭妯�(bi膩o)婧�(zh菙n)鍝佺窔鎬у洖姝告洸绶氾紝鎸夋洸绶氭柟绋嬭▓(j矛)绠楀悇妯ｆ湰婵冨害鍊笺��
鑷倷鐗╁搧
閰舵(bi膩o)鍎�锛�450nm锛�
楂樼簿搴﹀姞妯ｅ櫒鍙婃闋細0.5-10uL銆�2-20uL銆�20-200uL銆�200-1000uL
37鈩冩亽婧
鎿嶄綔娉ㄦ剰浜嬮爡(xi脿ng)
瑭﹀姂鐩掍繚瀛樺湪2-8鈩冿紝浣跨敤鍓嶅婧钩琛�20鍒嗛悩銆傚緸鍐扮鍙栧嚭鐨勬績绺礂婊屾恫鏈�(hu矛)鏈夌祼(ji茅)鏅讹紝閫欏爆浜庢甯哥従(xi脿n)璞★紝姘存荡鍔犵啽浣跨祼(ji茅)鏅�*婧惰В鍚庡啀浣跨敤銆�
瀵�(sh铆)椹�(y脿n)涓笉鐢ㄧ殑鏉挎鎳�(y墨ng)绔嬪嵆鏀惧洖鑷皝琚嬩腑锛屽瘑灏侊紙浣庢韩骞茬嚗锛変繚瀛樸��
婵冨害鐐�0鐨凷0铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸嵆鍙鐐洪櫚鎬у皪(du矛)鐓ф垨鑰呯┖鐧斤紱鎸夌収瑾�(shu艒)鏄庢浉鎿嶄綔鏅�(sh铆)妯ｆ湰宸茬稉(j墨ng)绋�閲�5鍊嶏紝绲傜祼(ji茅)鏋滀箻浠�5鎵嶆槸妯ｆ湰瀵�(sh铆)闅涙績搴︺��
鍤�(y谩n)鏍兼寜鐓ц(shu艒)鏄庢浉涓(bi膩o)鏄庣殑鏅�(sh铆)闁撱�佸姞娑查噺鍙婇爢搴忛��(j矛n)琛屾韩鑲叉搷浣溿��
鎵�鏈夋恫楂旂祫鍒嗕娇鐢ㄥ墠鍏呭垎鎼栧嫽銆�
瑭﹀姂鐩掔祫鎴�
鍚嶇ū96瀛旈厤缃�48瀛旈厤缃�鍌欐敞
寰瓟閰舵(bi膩o)鏉�12瀛斆�8姊�12瀛斆�4姊�鐒�(w煤)
妯�(bi膩o)婧�(zh菙n)鍝�0.3mL*6绠�0.3mL*6绠�鐒�(w煤)
妯ｆ湰绋�閲嬫恫6mL3mL鐒�(w煤)
妾㈡脯(c猫)鎶楅珨-HRP10mL5mL鐒�(w煤)
20脳娲楁粚绶╂矕娑�25mL15mL鎸夎(shu艒)鏄庢浉閫�(j矛n)琛岀█閲�
搴曠墿A6mL3mL鐒�(w煤)
搴曠墿B6mL3mL鐒�(w煤)
绲傛娑�6mL3mL鐒�(w煤)
灏佹澘鑶�2寮�2寮�鐒�(w煤)
瑾�(shu艒)鏄庢浉1浠�1浠�鐒�(w煤)
鑷皝琚�1鍊�(g猫)1鍊�(g猫)鐒�(w煤)
娉細妯�(bi膩o)婧�(zh菙n)鍝侊紙S0-S5锛夋績搴︿緷娆＄偤锛�0銆�12.5銆�25銆�50銆�100銆�200 mg/L
瑭﹀姂鐨勬簴(zh菙n)鍌�
20脳娲楁粚绶╂矕娑茬殑绋�閲嬶細钂搁ぞ姘存寜1锛�20绋�閲嬶紝鍗�1浠界殑20脳娲楁粚绶╂矕娑插姞19浠界殑钂搁ぞ姘淬��
娲楁澘鏂规硶
鎵嬪伐娲楁澘锛氱敥鐩″瓟鍏�(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛岄潨缃�1min鍚庣敥鐩″瓟鍏�(n猫i)娑查珨锛屽湪鍚告按绱欎笂鎷嶅共锛屽姝ゆ礂鏉�5娆°��
鑷嫊(d貌ng)娲楁澘姗�(j墨)锛氭瘡瀛旀敞鍏ユ礂娑�350渭L锛屾蹈娉�1min锛屾礂鏉�5娆°��
瑭﹀姂鐩掓�ц兘
婧�(zh菙n)纰烘�э細妯�(bi膩o)婧�(zh菙n)鍝佺窔鎬у洖姝歌垏闋�(y霉)鏈熸績搴︾浉闂�(gu膩n)绯绘暩(sh霉)R鍊硷紝澶т簬绛変簬0.9900銆�
闈堟晱搴︼細浣庢娓�(c猫)婵冨害灏忎簬1.0 mg/L銆�
鐗圭暟鎬э細涓嶈垏鍏跺畠鍙憾鎬х祼(ji茅)妲�(g貌u)椤炰技鐗╀氦鍙夊弽鎳�(y墨ng)銆�
閲嶅京(f霉)鎬э細鏉垮収(n猫i)銆佹澘闁撹畩鐣扮郴鏁�(sh霉)鍧囧皬浜�15%銆�
璨棌锛�2-8鈩冿紝閬垮厜闃叉疆淇濆瓨銆�
鏈夋晥鏈燂細6鍊�(g猫)鏈�
鍏嶈铂(z茅)鑱叉槑
瑭﹀姂鐩掑儏渚涚爺绌朵娇鐢紝涓嶅緱鐢ㄤ簬鑷ㄥ簥瀵�(sh铆)椹�(y脿n)鎴栦汉楂斿(sh铆)椹�(y脿n)锛屽惁鍓囨墍鐢�(ch菐n)鐢熺殑涓�鍒囧悗鏋滐紝鐢卞(sh铆)椹�(y脿n)鑰呮壙鎿�(d膩n)锛屾湰鍏徃姒備笉璨�(f霉)璨�(z茅)銆�
鍤�(y谩n)鏍兼寜鐓ц(shu艒)鏄庢浉鎿嶄綔锛屽(sh铆)椹�(y脿n)鑰呴仌鍙嶈(shu艒)鏄庢浉鎿嶄綔锛屽悗鏋滅敱瀵�(sh铆)椹�(y脿n)鑰呮壙鎿�(d膩n)銆�
- 01
- 02
SCHUNK闆勫厠姘ｇ几鐨勬媶鍗稿拰缍(h霉)姝ラ鏈夊摢浜�
- 03
ASCO涓栨牸闆荤闁ュ湪闆昏矾涓殑绗﹁櫉(h脿o)琛ㄧず
- 04
闆绘帶绯荤当(t菕ng)涓帶鍒朵俊铏�(h脿o)鍌宠几?sh霉)璋嬬鐥夋?/a>
- 05
鑷哀鑰佸寲瑭﹂(y脿n)绠遍亱(y霉n)琛岀洠(ji膩n)娓�(c猫)鑸囩暟甯搁熆鎳�(y墨ng)瑕�(gu墨)绋�
- 06
UNIVER闆荤闁ョ铏�(h脿o)瑭宠В锛氬伐浣滃師鐞嗚垏鎳�(y墨ng)鐢ㄤ竴绐虹┒绔�
- 07
- 08
ASCO闆荤闁ョ殑宸ヤ綔鍘熺悊銆佸瀷铏�(h脿o)鍙冩暩(sh霉)鍙婂収(n猫i)閮ㄧ祼(ji茅)妲�(g貌u)瑭宠В
- 09
SMC娓涘闁ユ�庝箞瑾�(di脿o)绡�(ji茅)澹撳姏澶у皬
- 10
FESTO姘ｇ几鐨勫叿楂旂祼(ji茅)妲�(g貌u)鍜屽伐浣滃師鐞�
- 11
绌嶅垎鎶界崕(ji菐ng)娲诲嫊(d貌ng)闁嬪鍟鏅鸿兘鍒堕�犵恫(w菐ng)鍠婁綘渚�(l谩i)闋�(l菒ng)閷�
鏅鸿兘鍒堕�犵恫(w菐ng)2024骞村害绌嶅垎鎶界崕(ji菐ng)娲诲嫊(d貌ng)鐝�(xi脿n)宸叉寮忛枊濮嬨�傛椿鍕�(d貌ng)鏅�(sh铆)闁撶偤2025骞�[瑭崇窗(x矛)]
- 鍑℃湰缍�(w菐ng)娉ㄦ槑"渚�(l谩i)婧愶細鏅鸿兘鍒堕�犵恫(w菐ng)"鐨勬墍鏈変綔鍝侊紝鐗堟瑠(qu谩n)鍧囧爆浜庢櫤鑳藉埗閫犵恫(w菐ng)锛岃綁(zhu菐n)杓夎珛(q菒ng)蹇呴爤娉ㄦ槑鏅鸿兘鍒堕�犵恫(w菐ng)锛宧ttp://www.lfljgfsj.com銆傞仌鍙嶈�呮湰缍�(w菐ng)灏囪拷绌剁浉闂�(gu膩n)娉曞緥璨�(z茅)浠汇��
- 浼佹キ(y猫)鐧�(f膩)甯冪殑鍏徃鏂拌仦銆佹妧琛�(sh霉)鏂囩珷銆佽硣鏂欎笅杓夌瓑鍏�(n猫i)瀹癸紝濡傛秹鍙婁镜娆�(qu谩n)銆侀仌瑕�(gu墨)閬姇瑷寸殑锛屼竴寰嬬敱鐧�(f膩)甯冧紒妤�(y猫)鑷鎵挎摂(d膩n)璨�(z茅)浠伙紝鏈恫(w菐ng)鏈夋瑠(qu谩n)鍒櫎鍏�(n猫i)瀹瑰苟杩芥函璨�(z茅)浠汇��
- 鏈恫(w菐ng)杞�(zhu菐n)杓夊苟娉ㄦ槑鑷叾瀹冧締(l谩i)婧愮殑浣滃搧锛岀洰鐨勫湪浜庡偝閬炴洿澶氫俊鎭紝骞朵笉浠ｈ〃鏈恫(w菐ng)璐婂悓鍏惰榛�(di菐n)鎴栬瓑瀵�(sh铆)鍏跺収(n猫i)瀹圭殑鐪熷(sh铆)鎬э紝涓嶆壙鎿�(d膩n)姝ら浣滃搧渚垫瑠(qu谩n)琛岀偤鐨勭洿鎺ヨ铂(z茅)浠诲強閫ｅ付璨�(z茅)浠汇�傚叾浠栧獟楂斻�佺恫(w菐ng)绔欐垨鍊�(g猫)浜哄緸鏈恫(w菐ng)杞�(zhu菐n)杓夋檪(sh铆)锛屽繀闋堜繚鐣欐湰缍�(w菐ng)娉ㄦ槑鐨勪綔鍝佷締(l谩i)婧愶紝骞惰嚜璨�(f霉)鐗堟瑠(qu谩n)绛夋硶寰嬭铂(z茅)浠汇��
- 濡傛秹鍙婁綔鍝佸収(n猫i)瀹广�佺増娆�(qu谩n)绛夊晱(w猫n)椤岋紝璜�(q菒ng)?ji膩n)璋ч珜閽抣(f膩)琛ㄤ箣鏃ヨ捣涓�鍛ㄥ収(n猫i)鑸囨湰缍�(w菐ng)鑱�(li谩n)绯伙紝鍚﹀墖瑕栫偤鏀炬鐩搁棞(gu膩n)娆�(qu谩n)鍒┿��
