璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sIgE)elisa瑭﹀姂鐩掓妧琛�(sh霉)鍙冩暩(sh霉)
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| 闂�(gu膩n) 閸� 瑭� | 璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sIgE)elisa瑭﹀姂鐩�,璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sI | ||
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璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sIgE)elisa瑭﹀姂鐩�瑾槑鏇�
瑭﹂(y脿n)鍘熺悊锛�
TRH瑭﹀姂鐩掓槸鍥虹浉澶惧績娉曢叾鑱�(li谩n)鍏嶇柅鍚搁檮瀵�(sh铆)椹�(y脿n)锛圗LISA锛�.宸茬煡TRH婵冨害鐨勬(bi膩o)婧�(zh菙n)鍝�銆佹湭鐭ユ績搴︾殑妯ｅ搧鍔犲叆寰瓟閰舵(bi膩o)鏉垮収(n猫i)閫�(j矛n)琛屾娓�(c猫)銆傚厛灏嘥RH鍜岀敓鐗﹕u妯�(bi膩o)瑷樼殑鎶楅珨鍚屾檪(sh铆)婧偛銆傛礂婊屽悗锛屽姞鍏ヨΚ鍜岀礌妯�(bi膩o)瑷橀亷鐨凥RP銆傚啀缍�(j墨ng)閬庢韩鑲插拰娲楁粚锛屽幓闄ゆ湭绲�(ji茅)鍚堢殑閰剁祼(ji茅)鍚堢墿锛岀劧鍚庡姞鍏ュ簳鐗〢銆丅锛屽拰閰剁祼(ji茅)鍚堢墿鍚屾檪(sh铆)浣滅敤銆傜敘(ch菐n)鐢熼鑹�銆傞鑹茬殑娣辨泛鍜屾ǎ鍝佷腑TRH鐨勬績搴﹀憟姣斾緥闂�(gu膩n)绯�銆�
瑭﹀姂鐩掑収(n猫i)瀹瑰強鍏堕厤鍒�
瑭﹀姂鐩掓垚浠斤紙2-8鈩冧繚瀛橈級 96瀛旈厤缃� 48瀛旈厤缃� 閰嶅埗
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璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sIgE)elisa瑭﹀姂鐩�濉戞枡鑶滄澘钃� 1濉� 鍗婂 鍗崇敤鍨�
妯�(bi膩o)婧�(zh菙n)鍝侊細24ng/ml 1鐡讹紙0.6ml锛� 1鐡讹紙0.3ml锛� 鎸夎鏄庢浉閫�(j矛n)琛岀█绋�
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妯�(bi膩o)婧�(zh菙n)鍝佺█閲嬬珐娌栨恫 1鐡讹紙5ml锛� 1鐡讹紙2.5ml锛� 鍗崇敤鍨�
鐢熺墿su妯�(bi膩o)瑷樼殑鎶桾RH鎶楅珨 1鐡讹紙6ml锛� 1鐡讹紙3.0ml锛� 鍗崇敤鍨�
瑕拰閺堥叾绱�-HRP 1鐡讹紙10ml锛� 1鐡讹紙5.0ml锛� 鍗崇敤鍨�
娲楁粚绶╂矕娑� 1鐡讹紙20ml锛� 1鐡讹紙10ml锛� 鎸夎鏄庢浉閫�(j矛n)琛岀█閲�
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搴曠墿B 1鐡讹紙6.0ml锛� 1鐡讹紙3.0ml锛� 鍗崇敤鍨�
绲傛娑� 1鐡讹紙6.0ml锛� 1鐡讹紙3.0ml锛� 鍗崇敤鍨�
鑷倷鏉愭枡
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2锛� 瀵�(sh铆)椹�(y脿n)涓笉瑕佸悆鍠濄�佹娊鐓欐垨浣跨敤鍖栧鍝�銆�
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鎿嶄綔娉ㄦ剰浜嬮爡(xi脿ng)
1锛� 瑭﹀姂鎳�(y墨ng)鎸夋(bi膩o)绨借鏄庢浉鍎�(ch菙)瀛�锛屼娇鐢ㄥ墠鎭㈠京(f霉)鍒板婧�銆傜█绋�閬庡悗鐨勬(bi膩o)婧�(zh菙n)鍝佹噳(y墨ng)涓熸锛屼笉鍙繚瀛�銆�
2锛� 瀵�(sh铆)椹�(y脿n)涓笉鐢ㄧ殑鏉挎鎳�(y墨ng)绔嬪嵆鏀惧洖鍖呰琚嬩腑锛屽瘑灏佷繚瀛橈紝浠ュ厤璁婅唱(zh矛)銆�
3锛� 涓嶇敤鐨勫叾瀹冭│鍔戞噳(y墨ng)鍖呰濂芥垨钃嬪ソ銆備笉鍚屾壒铏�(h脿o)鐨勮│鍔戜笉瑕佹贩鐢�銆備繚璩�(zh矛)鍓嶄娇鐢ㄣ��
4锛� 浣跨敤涓�娆℃�х殑鍚搁牠浠ュ厤浜ゅ弶姹℃煋锛屽惛鍙栫祩姝㈡恫鍜屽簳鐗〢銆丅娑叉檪(sh铆)锛岄伩鍏嶄娇鐢ㄥ付閲戝爆閮ㄥ垎鐨勫姞妯ｅ櫒銆�
5锛� 浣跨敤骞插噲鐨勫鏂欏鍣ㄩ厤缃礂婊屾恫銆備娇鐢ㄥ墠鍏呭垎娣峰嫽瑭﹀姂鐩掗噷鐨勫悇绋垚浠藉強妯ｅ搧銆�
6锛� 娲楁粚閰舵(bi膩o)鏉挎檪(sh铆)鎳�(y墨ng)鍏呭垎鎷嶅共锛屼笉瑕佸皣鍚告按绱欑洿鎺ユ斁鍏ラ叾妯�(bi膩o)鍙嶆噳(y墨ng)瀛斾腑鍚告按銆�
7锛� 搴曠墿A鎳�(y墨ng)鎻櫦(f膩)锛岄伩鍏嶉暦(zh菐ng)鏅�(sh铆)闁撴墦闁嬭搵瀛�銆傚簳鐗〣灏�(du矛)鍏夋晱鎰�锛岄伩鍏嶉暦(zh菐ng)鏅�(sh铆)闁撴毚闇蹭簬鍏変笅銆傞伩鍏嶇敤鎵嬫帴瑙�锛屾湁姣�銆傚(sh铆)椹�(y脿n)瀹屾垚鍚庢噳(y墨ng)绔嬪嵆璁�鍙朞D鍊�銆�
8锛� 鍔犲叆瑭﹀姂鐨勯爢搴忔噳(y墨ng)涓�鑷�锛屼互淇濊瓑鎵�鏈夊弽鎳�(y墨ng)鏉垮瓟婧偛鐨勬檪(sh铆)闁撲竴妯ｃ��
9锛� 鎸夌収瑾槑鏇镐腑妯�(bi膩o)鏄庣殑鏅�(sh铆)闁撱�佸姞娑茬殑閲忓強闋嗗簭閫�(j矛n)琛屾韩鑲叉搷浣�銆�
璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sIgE)elisa瑭﹀姂鐩�妯ｅ搧鏀堕泦銆佽檿鐞嗗強淇濆瓨鏂规硶
1銆� 琛�娓�-----鎿嶄綔閬庣▼涓伩鍏嶄换浣曠窗(x矛)鑳炲埡婵�銆備娇鐢ㄤ笉鍚啽鍘熷拰鍏�(n猫i)姣掔礌鐨勮│绠°�傛敹闆嗚娑插悗锛�1000脳g闆㈠績10鍒嗛悩灏囪娓呭拰绱呯窗(x矛)鑳炶繀閫熷皬蹇冨湴鍒嗛洟銆�
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3銆� 绱�(x矛)鑳炰笂娓呮恫---1000脳g闆㈠績10鍒嗛悩鍘婚櫎椤嗙矑鍜岃仛鍚堢墿銆�
4銆� 淇濆瓨------濡傛灉妯ｅ搧涓嶇珛鍗充娇鐢�锛屾噳(y墨ng)灏囧叾鍒嗘垚灏忛儴鍒�-70 鈩冧繚瀛�锛岄伩鍏嶅弽寰�(f霉)鍐峰噸銆傜洝鍙兘鐨勪笉瑕佷娇鐢ㄦ憾琛�鎴栭珮琛�鑴傝銆傚鏋滆娓呬腑澶ч噺椤嗙矑锛屾娓�(c猫)鍓嶅厛闆㈠績鎴栭亷婵俱�備笉瑕佸湪37鈩冩垨鏇撮珮鐨勬韩搴﹀姞鐔辫В鍑�銆傛噳(y墨ng)鍦ㄥ婧笅瑙ｅ噸骞剁⒑淇濇ǎ鍝佸潎鍕诲湴鍏呭垎瑙ｅ噸銆�
瑭﹀姂鐨勬簴(zh菙n)鍌�
1锛� 妯�(bi膩o)婧�(zh菙n)鍝侊細妯�(bi膩o)婧�(zh菙n)鍝佺殑绯诲垪绋�閲嬫噳(y墨ng)鍦ㄥ(sh铆)椹�(y脿n)鏅�(sh铆)婧�(zh菙n)鍌欙紝涓嶈兘鍎�(ch菙)瀛樸�傜█閲嬪墠灏囨(bi膩o)婧�(zh菙n)鍝佹尟钑╂贩鍕�銆傜█閲嬫瘮渚嬫寜涓嬭〃涓��(j矛n)琛岋細
24 ng/ml 锛�6铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 鍘熷�嶆績搴︿笉鐢ㄧ█閲嬬洿鎺ュ姞鍏�50ul銆�
12 ng/ml 锛�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨勫師鍊嶆(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
6.0 ng/ml 锛�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�5铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
3.0 ng/ml 锛�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�4铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
1.5 ng/ml 锛�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�3铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
0.75 ng/ml 锛�1铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝侊級 100ul鐨�2铏�(h脿o)妯�(bi膩o)婧�(zh菙n)鍝佸姞鍏�100ul鐨勬(bi膩o)婧�(zh菙n)鍝佺█閲嬫恫
0 ng/ml 锛堢┖鐧藉皪(du矛)鐓э級 鍘熷婵冨害涓嶇敤绋�閲嬬洿鎺ュ姞鍏�50ul銆�
2锛� 娲楁粚绶╂矕娑诧紙50脳锛夌殑绋�閲嬶細钂搁ぞ姘�50鍊嶇█閲�銆�
鎿嶄綔姝ラ
1锛� 浣跨敤鍓嶏紝灏囨墍鏈夎│鍔戝厖鍒嗘贩鍕�銆備笉瑕佷娇娑查珨鐢�(ch菐n)鐢熷ぇ閲忕殑娉℃搏锛屼互鍏嶅姞妯ｆ檪(sh铆)鍔犲叆澶ч噺鐨勬埃娉★紝鐢�(ch菐n)鐢熷姞妯ｄ笂鐨勮宸�銆�
2锛� 鏍规摎(j霉)寰呮脯(c猫)妯ｅ搧鏁�(sh霉)閲忓姞涓婃(bi膩o)婧�(zh菙n)鍝佺殑鏁�(sh霉)閲忔焙瀹氭墍闇�鐨勬澘姊濇暩(sh霉)銆傛瘡鍊�(g猫)妯�(bi膩o)婧�(zh菙n)鍝佸拰绌虹櫧瀛斿缓璀板仛寰�(f霉)瀛�銆傛瘡鍊�(g猫)妯ｅ搧鏍规摎(j霉)鑷繁鐨勬暩(sh霉)閲忎締瀹�锛岃兘浣跨敤寰�(f霉)瀛旂殑鐩￠噺鍋氬京(f霉)瀛�銆�
3锛� 鍔犲叆绋�閲嬪ソ鍚庣殑妯�(bi膩o)婧�(zh菙n)鍝�50ul浜庡弽鎳�(y墨ng)瀛�銆佸姞鍏ュ緟娓�(c猫)妯ｅ搧50ul浜庡弽鎳�(y墨ng)瀛斿収(n猫i)銆傜珛鍗冲姞鍏�50ul鐨勭敓鐗﹕u妯�(bi膩o)瑷樼殑鎶楅珨銆傝搵涓婅啘鏉�锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�1灏忔檪(sh铆)銆�
4锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉�锛岀敥鍘绘礂婊屾恫锛岀敤鍚告按绱欐媿骞�銆傞噸寰�(f霉)姝ゆ搷浣�3娆�銆傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆�銆�
5锛� 姣忓瓟鍔犲叆80ul鐨勮Κ鍜岄張閰剁礌-HRP锛岃紩杓曟尟钑╂贩鍕�锛�37鈩冩韩鑲�30鍒嗛悩銆�
6锛� 鐢╁幓瀛斿収(n猫i)娑查珨锛屾瘡瀛斿姞婊挎礂婊屾恫锛屾尟钑�30绉�锛岀敥鍘绘礂婊屾恫锛岀敤鍚告按绱欐媿骞�銆傞噸寰�(f霉)姝ゆ搷浣�3娆�銆傚鏋滅敤娲楁澘姗�(j墨)娲楁粚锛屾礂婊屾鏁�(sh霉)澧炲姞涓�娆�銆�
7锛� 姣忓瓟鍔犲叆搴曠墿A銆丅鍚�50ul锛岃紩杓曟尟钑╂贩鍕伙紝37鈩冩韩鑲�10鍒嗛悩銆傞伩鍏嶅厜鐓�銆�
8锛� 鍙栧嚭閰舵(bi膩o)鏉�锛岃繀閫熷姞鍏�50ul绲傛娑�锛屽姞鍏ョ祩姝㈡恫鍚庢噳(y墨ng)绔嬪嵆娓�(c猫)瀹氱祼(ji茅)鏋�銆�
9锛� 鍦�450nm娉㈤暦(zh菐ng)铏曟脯(c猫)瀹氬悇瀛旂殑OD鍊�銆�
寤鸿浣跨敤鐨勫(sh铆)椹�(y脿n)鏂规
璞氶紶鍗垫竻铔嬬櫧鐗圭暟鎬gE(OVA sIgE)elisa瑭﹀姂鐩� 妯�(bi膩o)婧�(zh菙n)鍝佹績搴︼紙ng/ml锛�
A 24 24 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
B 12 12 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
C 6.0 6.0 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
D 3.0 3.0 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
E 1.5 1.5 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
F 0.75 0.75 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
G 0 0 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
H 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧 妯ｅ搧
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