Anti-Bax antibody [6A7] ab5714
12 Abreviews 16 References 4 圖像
Product name Anti-Bax抗體[6A7]
Description 小鼠單克隆抗體[6A7] to Bax
Tested applications Flow Cyt, ICC/IF, IP, WB
Species reactivity Reacts with: Mouse, Rat, Human, Chinese Hamster
Immunogen
Run BLAST with Run BLAST with
Synthetic peptide corresponding to Human Bax aa 12-24 (N terminal) conjugated to Keyhole Limpet
Haemocyanin (KLH) (Cysteine residue).
Sequence:
CGPTSSEQIMKTGA
Database link: Q07812
Form Liquid
Storage instructions Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid
freeze / thaw cycle.
Storage buffer pH: 8.20
Constituents: 0.09% Sodium borate, 0.09% Sodium chloride
Sodium Borate and Sodium Chloride are used to produce a Borate-buffered saline.
Purity Protein G purified
Clonality Monoclonal
Clone number 6A7
Isotype IgG1
Overview
Properties
Applications
Our Abpromise guarantee covers the use of ab5714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
Flow Cyt 1/10. Ab81216-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 μg/ml. PubMed: 14766748Use at a concentration of 5 μg/ml (see Gardai et al
reference).
IP Use at 2 μg/mg of lysate. PubMed: 14522999Use at an assay dependent dilution (see Yethon et al
reference).
WB Use a concentration of 1 - 2 μg/ml. Detects a band of approximay 20-22 kDa.
Target
Product Datasheet
http://www.abcam。。ｃｎ/Bax-antibody-6A7-ab5714.html 1
in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic
leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung
carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
Sequence similarities Belongs to the Bcl-2 family.
Domain Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their
interaction with anti-apoptotic members of the Bcl-2 family.
Cellular localization Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under
stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3
proteins and translocation to the mitochondrion membrane.
Anti-Bax antibody [6A7] images
Immunocytochemistry/ Immunofluorescence - Anti-
Bax antibody [6A7] (ab5714)
This image is courtesy of an Abreview submitted by Dr
Alwin Scharstuhl
Immunofluorescence analysis of Human foreskin
fibroblast cells, staining Bax with ab5714.
Cells were fixed with paraformaldehyde, permeabilized
with 0.1% saponin prior to being blocked in 1% BSA + 2%
normal goat serum for 30 mins at 20°C. Samples were
incubated with 5 μg/ml primary antibody for 45 mins at
20°C; the diution buffer was 1% BSA, 0.1% saponin,
0.05% NaN3 in PBS. An Alexa Fluor? 594-conjugated Goat
polyclonal to mouse IgG (ab150116), dilution 1/1000, was
used as secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-
Bax antibody [6A7] (ab5714)
This image is courtesy of an anonymous Abreview
Immunofluorescence analysis of Human PMN cells
staining Bax with ab5714.
The cells were fixed in paraformaldehyde, permeabilised
in 0.1% Triton X-100 and then blocked using 2% BSA for 1
hour at 22°C. Samples were then incubated with primary
antibody at 1/200 for 16 hours at 4°C. The secondary
antibody used was a goat anti-mouse IgG (H+L)
conjugated to Alexa Fluor? 488 (green) (ab150113) used
at a 1/500 dilution. Counterstained with DAPI (blue).
Product Datasheet
http://www.abcam。。ｃｎ/Bax-antibody-6A7-ab5714.html 2
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-Bax antibody [6A7] (ab5714)
Image courtesy of an anonymous Abreview.
Anti-Bax antibody [6A7] (ab5714) at 1/2000 dilution +
whole tissue lysate prepared from human islet tissue at
20 μg
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/10000
dilution
developed using the ECL technique
Observed band size : 20 kDa
Additional bands at : 35 kDa. We are unsure as to the
identity of these extra bands.
Exposure time : 30 seconds
Image courtesy of an anonymous Abreview.
Flow Cytometry-Bax antibody [6A7](ab5714)
Overlay histogram showing HeLa cells stained with
ab5714 (red line). The cells were fixed with 4%
paraformaldehyde (10 min) and then permeabilized with
0.1% PBS-Tween for 20 min. The cells were then
incubated in 1x PBS / 10% normal goat serum / 0.3M
glycine to block non-specific protein-protein interactions
followed by the antibody (ab5714, 1/10 dilution) for 30 min
at 22°C. The secondary antibody used was DyLight? 488
goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for
30 min at 22°C. Isotype control antibody (black line) was
mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used
under the same conditions. Acquisition of >5,000 events
was performed. This antibody gave a positive signal in
HeLa cells fixed with methanol (5 min)/permeabilized in
0.1% PBS-Tween used under the same conditions.
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